Establishment of Quantitative PCR Assays for Active Long Interspersed Nuclear Element-1 Subfamilies in Mice and Applications to the Analysis of Aging-Associated Retrotransposition

Kuroki, Ryota and Murata, Yui and Fuke, Satoshi and Nakachi, Yutaka and Nakashima, Jun and Kujoth, Gregory C. and Prolla, Tomas A. and Bundo, Miki and Kato, Tadafumi and Iwamoto, Kazuya (2020) Establishment of Quantitative PCR Assays for Active Long Interspersed Nuclear Element-1 Subfamilies in Mice and Applications to the Analysis of Aging-Associated Retrotransposition. Frontiers in Genetics, 11. ISSN 1664-8021

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Abstract

The retrotransposon long interspersed nuclear element-1 (LINE-1) can autonomously increase its copy number within a host genome through the retrotransposition process. LINE-1 is active in the germline and in neural progenitor cells, and its somatic retrotransposition activity has a broad impact on neural development and susceptibility to neuropsychiatric disorders. The method to quantify the genomic copy number of LINE-1 would be important in unraveling the role of retrotransposition, especially in the brain. However, because of the species-specific evolution of LINE-1 sequences, methods for quantifying the copy number should be independently developed. Here, we developed a quantitative PCR (qPCR) assay to measure the copy number of active LINE-1 subfamilies in mice. Using the assay, we investigated aging-associated alterations of LINE-1 copy number in several brain regions in wild-type mice and Polg+/D257A mice as a model for accelerated aging. We found that aged Polg+/D257A mice showed higher levels of the type GfII LINE-1 in the basal ganglia than the wild-type mice did, highlighting the importance of assays that focus on an individual active LINE-1 subfamily.

Item Type: Article
Subjects: Lib Research Guardians > Medical Science
Divisions: Faculty of Engineering, Science and Mathematics > School of Physics
Depositing User: Unnamed user with email support@lib.researchguardians.com
Date Deposited: 02 Feb 2023 12:19
Last Modified: 27 Jul 2024 13:19
URI: http://eprints.classicrepository.com/id/eprint/82

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