Ozonated Water Decreases In vitro Contamination at Dental Implant Interface in Different Connections

Aim: Cases of peri-implant diseases have increased, highlighting the need for preventive and therapeutic protocols. This study aimed to evaluate the effectiveness of ozonated water on In vitro decontamination of implant-abutment interfaces in external hexagon (EH) and morse cone (MC) connections.

Study Design: In vitro study.

Place and Duration of Study: Sample: Department of Periodontics and Implant Dentistry of Ingá University Center – UNINGÁ, between June 2020 and December 2021.

Methodology: Twenty-four implant-abutments (IA) in EH (n=12) and MC (n=12) connections were divided into groups: negative control (NC) – sterile IA; positive control (PC) – IA contaminated with biofilm; and ozone (O) – IA contaminated with biofilm and decontaminated with ozonated water (60µg/mL,1min). The effectiveness of ozonated water was evaluated by counting colony-forming units (CFU/mL), and both connection types were compared.

Results: There was a significant difference among groups in both connection types, outside and inside the implants (P=0.000). Group O showed a significant decrease in CFU/mL compared to group PC outside and inside the implants for EH and MC connections (P=0.000). Only group O in the EH connection presented a significative difference in CFU/mL compared to outside and inside the implants (2.475x103±0.320 and 1.775x103±0.125, respectively) (P=0.033). Comparison between connections was statistically different for groups PC and O, outside and inside the implants (P=0.000).

Conclusion: Ozonated water showed effectiveness in vitro decontamination of implant-abutment interfaces in both connection types.