Singh, Rajender and Jat, Ranjeet Singh and Srinivasan, . (2002) Agrobacterium-mediated Genetic Transformation of Chickpea (Cicer arietinum L.). PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, 3 (3-4). pp. 125-130.
Full text not available from this repository.Abstract
Transformation of chickpea (Cicer arietinum L.) was achieved using Agrobacterium tumefaciens strain EHA105 harboring binary vector p35SGUSINT containing uidA (GUS) and npt II genes, and pJB90GI containing uid A (GUS) and hpt genes. A strong GUS expression was detected in the putative transformed shoots by the histochemical assay. Transformation was confirmed by the presence of 0.69kb and 0.82kb amplified fragments for npt II and hpt genes, respectively, by PCR analysis. Multiple shoot formation was achieved in cotyledonary notch region on MS medium supplemented with 1.0 mM thidiazuron.
| Item Type: | Article |
|---|---|
| Subjects: | Lib Research Guardians > Biological Science |
| Depositing User: | Unnamed user with email support@lib.researchguardians.com |
| Date Deposited: | 06 Dec 2023 11:47 |
| Last Modified: | 11 Jul 2024 04:46 |
| URI: | http://eprints.classicrepository.com/id/eprint/2391 |
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