Opposite Effects of F1 and F5 Fractions of Total Methanol Leaf-extract of Dialium guineense (Cesalpiniaceae) on Blood Glucose in Rat

Barboza, Firmin Sylva and Sène, Madièye and Doupa, Dominique and Ba, Malick and Wélé, Alassane and Yoro, Sy Guata (2020) Opposite Effects of F1 and F5 Fractions of Total Methanol Leaf-extract of Dialium guineense (Cesalpiniaceae) on Blood Glucose in Rat. Journal of Advances in Medical and Pharmaceutical Sciences, 22 (2). pp. 1-8. ISSN 2394-1111

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Abstract

The purpose of this study is to investigate the effects of total methanol leaf-extract of Dialium guineense (Cesalpiniaceae) and its fractions on blood glucose in rat.

The fractions of methanol extract were obtained by chromatography Sephadex LH 20 gel, numbered F1 to F5. Experiments were performed in normoglycemic, glucose tolerance test, and type 2 diabetic rats.

The total methanol leaf-extract (300 mg/kg, per os), induced a significant increase of blood glucose level in normoglycemic rats (2.27±0.12 vs 0.94±0.03 g/L) (p<0.05, n=5). F1 and F2 fractions (100 mg/kg, per os) increased the blood glucose level. Glycaemia respectively varied from 0.90 ± 0.03 to 2. 28 ± 0.22 g/L and 0.91 ± 0.03 to 1.43 ± 0.04 g /L (p<0.05, n=5). However, F5 fraction (300 mg/kg, per os), induced hypoglycemia (0.61 ± 0.01 vs 0. 80 ± 0.03 g/L) (p<0.05, n=5). F5 fraction prevented the pic of hyperglycemia caused by glucose (4 g/kg, per os). In type 2 diabetic rats, the daily oral administration of F5 fraction (300 mg/kg) induced an anti-hyperglycemic effect (1.28 ± 0.15 vs 4.48 ± 0.08 g/L). Fractionation by gel permeation chromatography allowed to highlight the presence of compounds both hyper-and hypoglycemic in total methanol leaf-extract of D. guineense. The lack of hypoglycemic effect in normoglycemic rats of total methanol leaf-extract, could respectively be related to the presence of both hyper- and hypoglycemic compounds in F1 and F5 fractions, which induced opposite effects.

Item Type: Article
Subjects: Lib Research Guardians > Medical Science
Depositing User: Unnamed user with email support@lib.researchguardians.com
Date Deposited: 23 Feb 2023 11:32
Last Modified: 10 Jul 2024 05:42
URI: http://eprints.classicrepository.com/id/eprint/231

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