An Isocratic Water Mobile Phase HPLC Analysis of Melamine and Its Related Analogues

The author describes a reserved-phase high performance liquid chromatographic method for
detecting melamine (MEL) and related analogues, cyanuric acid (CYA), ammeline (AML), and
ammelide (AMD), using a 100% water mobile phase. Chromatographic separation was performed an
Inertsil® ODS-4 (250 × 4.6 mm, 5 μm) with a water mobile phase and a photodiode-array detector.
The monitoring wavelength was adjusted to 210 nm which represents an average maximum for all the
analytes. The total run time was < 8 min. The method shows high stability, significant linearity and
satisfactory sensitivity. The detection limits were <46 ng.mL–1. An inexpensive and harmless method
for the simultaneous detection of MEL, CYA, AML, and AMD was developed and may be further
applied to the quantification in foods